Pleural 9 Neuron

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Pleural 9 is a neuron in Tritonia.

Basic Information

Injection of 5,6-carboxyfluorescein illuminates the size and location of the Pl 9 cell in the lateral region of the dorsal pleural ganglion. Pd, Pedal ganglion; Ce, Cerebral ganglion; Pl, Pleural ganglion (Frost et al., 2003).
Color image from Lee AH, Megalou EV, Wang J, Frost WN. Axonal conduction block as a novel mechanism of prepulse inhibition. J Neurosci. 2012 Oct 31;32(44):15262-70.

Identification

Anatomy

  • The Pl 9 neuron type refers to one or two 70 micron cells located one or two cell layers below the dorsal surface of the pleural ganglion on each side of the brain.
  • Pl 9 is situated close to the region where Pleural Nerve 1 enters the ganglion.
Pl9 and its synaptic connectivity to Tritonia escape swim circuit. Bars correspond to excitatory connections, black circles to inhibitory synaptic connections, bars and circles correspond to multiple component PSPs. Presynaptic inhibition occurs where Pl 9 inhibitory synapses occur during S cell excitatory synapses (Frost et al., 2003).


Electrophysiology

  • Pl 9 is known to receive direct EPSPs from most or all of the ipsilateral S-cells and conversely, it produces fast IPSPs onto the entire S cell population.
  • Pl 9 can be identified in an isolated brain preparation by first impaling one or more S-cells with a microelectrode and then probing the cells below with another microelectrode for a cell that receives monosynaptic EPSPs from the S-cells and produces one-for-one fast IPSPs onto the S-cells.
  • Pleural 9 produces direct IPSPs onto the TR1, DRI and VSI-B interneurons.
  • Pl 9 is known to play a role in mediating prepulse inhibition (PPI) in Tritonia. Intracellular stimulation of Pl 9 mimics the inhibitory effects of skin prepulses on the S cell activity. Pl 9 can block the swim motor program, partly due to the cell's ability to reduce the efficacy of the excitatory S cell synapse onto the interneurons that initiate the stereotypic Tritonia escape response.

Method for identifying Pl9

from Lee et al (2012)

  1. Place an intracellular electrode in an S-cell.
  2. With a second electrode blindly penetrate neurons one cell layer below the surface at the known location of Pl-9
  3. It can be identified by its production of 1-for-1, short and constant latency IPSPs onto the S-cell with each action potential, and its receipt of 1-for-1 short and constant latency EPSPs from ipsilateral S-cells.

References

  • Frost WN, Tian LM, Hoppe TA, Mongeluzi DL, Wang J. A cellular mechanism for prepulse inhibition. Neuron. 2003 Dec 4;40(5):991-1001. PubMed ID: 14659097. [1]
  • Lee AH, Megalou EV, Wang J, Frost WN. Axonal conduction block as a novel mechanism of prepulse inhibition. J Neurosci. 2012 Oct 31;32(44):15262-70. [2]